File:A-conserved-leucine-occupies-the-empty-substrate-site-of-LeuT-in-the-Na+-free-return-state-ncomms11673-s3.ogv
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A-conserved-leucine-occupies-the-empty-substrate-site-of-LeuT-in-the-Na+-free-return-state-ncomms11673-s3.ogv (Ogg Theora video file, length 20 s, 640 × 480 pixels, 2.76 Mbps, file size: 6.66 MB)
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[edit]DescriptionA-conserved-leucine-occupies-the-empty-substrate-site-of-LeuT-in-the-Na+-free-return-state-ncomms11673-s3.ogv |
English: Supplementary Movie 2 The movie depicts the full transport cycle of the NSS family going from the outward-facing return state (presented here) through all known states of the NSS cycle (see supplementary discussion and supplementary figure 6 for further details). The movie 'morphs' between available structures - LeuT outward-facing, Na+-free return state (this study), LeuT outward-open, Na+-bound1 (PDB entry code 3TT1), LeuT outward-oriented substrate-occluded (PDB entry code 2A65), MhsT inward-oriented and substrate-occluded (PDB entry code 4US3), and LeuT inward-open apoform (PDB entry code 3TT3). For compatibility in morphing procedures, a leucine and sodium-bound inward-oriented and occluded state was modeled for LeuT based on the MhsT structure as described in Methods. The structures were aligned on the scaffold domain (TMs 3-4, 8-9). TM5 is shown as a cyan helix, TM1 as red helix, Na+ ions at Na1 and Na2 sites are shown in green, leucine in orange. |
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Source | Video file from Malinauskaite L, Said S, Sahin C, Grouleff J, Shahsavar A, Bjerregaard H, Noer P, Severinsen K, Boesen T, Schiøtt B, Sinning S, Nissen P (2016). "A conserved leucine occupies the empty substrate site of LeuT in the Na+-free return state". Nature Communications. DOI:10.1038/ncomms11673. PMID 27221344. PMC: 4894957. | ||
Author | Malinauskaite L, Said S, Sahin C, Grouleff J, Shahsavar A, Bjerregaard H, Noer P, Severinsen K, Boesen T, Schiøtt B, Sinning S, Nissen P | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 19:46, 27 October 2016 | 20 s, 640 × 480 (6.66 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Supplementary Movie 2 |
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Author | Malinauskaite L, Said S, Sahin C, Grouleff J, Shahsavar A, Bjerregaard H, Noer P, Severinsen K, Boesen T, Schiøtt B, Sinning S, Nissen P |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | The movie depicts the full transport cycle of the NSS family going from the outward-facing return state (presented here) through all known states of the NSS cycle (see supplementary discussion and supplementary figure 6 for further details). The movie 'morphs' between available structures - LeuT outward-facing, Na+-free return state (this study), LeuT outward-open, Na+-bound1 (PDB entry code 3TT1), LeuT outward-oriented substrate-occluded (PDB entry code 2A65), MhsT inward-oriented and substrate-occluded (PDB entry code 4US3), and LeuT inward-open apoform (PDB entry code 3TT3). For compatibility in morphing procedures, a leucine and sodium-bound inward-oriented and occluded state was modeled for LeuT based on the MhsT structure as described in Methods. The structures were aligned on the scaffold domain (TMs 3-4, 8-9). TM5 is shown as a cyan helix, TM1 as red helix, Na+ ions at Na1 and Na2 sites are shown in green, leucine in orange. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2016-05-25 |