File:A-major-secretory-defect-of-tumour-infiltrating-T-lymphocytes-due-to-galectin-impairing-LFA-1-ncomms12242-s2.ogv
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A-major-secretory-defect-of-tumour-infiltrating-T-lymphocytes-due-to-galectin-impairing-LFA-1-ncomms12242-s2.ogv (Ogg Theora video file, length 6.7 s, 130 × 130 pixels, 116 kbps, file size: 94 KB)
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[edit]DescriptionA-major-secretory-defect-of-tumour-infiltrating-T-lymphocytes-due-to-galectin-impairing-LFA-1-ncomms12242-s2.ogv |
English: Supplementary Movie 1 Monitoring of untreated TIL conjugates under flow stress conditions. Untreated CD8 TILs from patient LB3450 (Supplementary Table 1) were stained with CFSE (green) and allowed to interact for 30 min with CMTMR (red)-loaded sAg-pulsed B cells. A flow of prewarmed medium was generated by progressively increasing the pressure up to 2 bar, by steps of 0.1 bar. The movie starts at 0.5 bar. TIL detachment from their targets was monitored by video microscopy. Scale bar represents 10 μm. Frames were collected at 4- second intervals. Display rate: 15 frames/second. |
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Source | Video file from Petit A, Demotte N, Scheid B, Wildmann C, Bigirimana R, Gordon-Alonso M, Carrasco J, Valitutti S, Godelaine D, van der Bruggen P (2016). "A major secretory defect of tumour-infiltrating T lymphocytes due to galectin impairing LFA-1-mediated synapse completion". Nature Communications. DOI:10.1038/ncomms12242. PMID 27447355. PMC: 4961845. | ||
Author | Petit A, Demotte N, Scheid B, Wildmann C, Bigirimana R, Gordon-Alonso M, Carrasco J, Valitutti S, Godelaine D, van der Bruggen P | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 19:56, 26 October 2016 | 6.7 s, 130 × 130 (94 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Supplementary Movie 1 |
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Author | Petit A, Demotte N, Scheid B, Wildmann C, Bigirimana R, Gordon-Alonso M, Carrasco J, Valitutti S, Godelaine D, van der Bruggen P |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Monitoring of untreated TIL conjugates under flow stress conditions. Untreated CD8 TILs from patient LB3450 (Supplementary Table 1) were stained with CFSE (green) and allowed to interact for 30 min with CMTMR (red)-loaded sAg-pulsed B cells. A flow of prewarmed medium was generated by progressively increasing the pressure up to 2 bar, by steps of 0.1 bar. The movie starts at 0.5 bar. TIL detachment from their targets was monitored by video microscopy. Scale bar represents 10 μm. Frames were collected at 4- second intervals. Display rate: 15 frames/second. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2016-07-22 |